منابع مشابه
Vitamin D receptor mediates DNA repair and is UV-inducible in intact epidermis but not cultured keratinocytes
While providing a powerful approach for studying epidermal biology, cultured keratinocytes may imperfectly model a three-dimensional epidermis in which cells are architecturally ordered. We report two important examples of the limitations of cultured keratinocytes in understanding vitamin D receptor (VDR) photobiology in murine skin. Recently, the vitamin D signaling pathway has been implicated...
متن کاملIntragenomal distribution of DNA repair synthesis: repair in satellite and mainband DNA in cultured mouse cells.
DNA repair synthesis was examined in mouse satellite and mainband DNA derived from confluent Balb/c 3T3 cells damaged with ultraviolet radiation or N-acetoxy-2-acetylaminofluorene. Two different approaches were used: (i) Contact-inhibited cells were treated with hydroxyurea to reduce replicative synthesis to low levels; and (ii) bromodeoxyuridine was used to label newly replicated DNA in cells ...
متن کاملComplementation assays adapted for DNA repair-deficient keratinocytes.
Genetic alterations affecting nucleotide excision repair, the most versatile DNA-repair mechanism responsible for removal of bulky DNA adducts including ultraviolet (UV) light-induced DNA lesions, may result in the rare, recessively inherited autosomal syndromes xeroderma pigmentosum (XP), Cockayne syndrome (CS), or trichothiodystrophy (TTD). Classical approaches such as somatic cell fusions or...
متن کاملDNA-Repair Capacity in Down\'s Syndrome
Down's syndrome (DS) is the most common chromosomal abnormality in human. Subjects with DS are known to be peridisposed to develop leukemia. The molecular basis of the association between DS and leukemia is unknown. The unscheduled DNA synthesis (UDS) test measure the ability of DNA-repair in mammalian cells after excision of a stretch of DNA containing the region of damage induced by chemical ...
متن کاملCultured Normal Human Epidermal Keratinocytes
H]glucosamine has been used to label metabolically keratinocyte cell-surface glycoconjugates. The major labelled bands identified on sodium dodecyl sulphate/polyacrylamide gels had apparent molecular weights of greater than 250000, and 150000-80000. Most of these components were trypsin-sensitive, indicating that the label was protein-bound. Some of the labelled components were shown to be prot...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 1983
ISSN: 0022-202X
DOI: 10.1111/1523-1747.ep12541076